BioTech Bootcamp Summer 2010 Tentative

8:00 AMWelcome (Classroom)Recap of Monday & questionsRecap of Tuesday & questionsRecap of Wednesday & questionsRecap of Thursday & questionsClassroom Recap of last week
Planning & Documenting Laboratory Dealing with complicated protocols-Practical dilution exercise. -Plating bacteria to get a concentration.Growing bacterial cellsProtein purification – GPF from pGLO transformed cellsStudent Assessment of Biotech Bootcamp
9:00 AMRegistration (auditorium)Associate Company roundtable discussion
Measuring, adjusting and controlling pHG-MOD lab II
10:00 AMTour of laboratory and floor (pix taken while on tour)Using laboratory balancesCentrifugation exercise
-Measuring pH of a liquid -Proper use of a pH meter -Calibrating a pH meter
Reproducibility and Accuracy of Measurements – Data Analysis Using ExcelBacterial plasmid miniprepProtein assay – colorimetricResearch Lab roundatable discussions
11:00 AMIntroduction and general orientation
Buffers – What are they and why are they used?Spectrophotometry (cont.)
General laboratory safety
12:00 PMLunchLunchLunchLunchLunchLunch
1:00 PMLaboratory safety exerciseNon-sterile vs. sterile techniquesBuffers – comparison of titrating a salt solution and a bufferTransformationEnzyme catalysisInterns report to their respective companies
Preparation of LB broth and LB agar
-Reading MSDS sheets -Finding safety information on chemical bottles
2:00 PMPreparation of Glycine/Mg buffer (pH 7.0, pH 8.0, pH 9.0 & pH 10.0) Preparation of PNPPG-MOD lab I
Handling and measuring liquids II. Stock solutions and dilutions
3:00 PMUnits and the metric system
-Preparing 1X TAE -Serial two-fold dilutionsSpectrophotometeryLab Math IV -Representing data -Semi-log graph paper -Determining rates
Handling & measuring liquids I. Pipets and other measuring devicesHow to prepare Powerpoint material: A Powerpoint tutorial for posters and presentations
4:00 PM
Reading a scientific paperReading a scientific paper (Part II)Reading a scientific paper (Part III)
Rainbow pipeting lab